SC106 Mycotoxin Aflatoxin M1 Rapid Test Kit(0.03ppb)

Aflatoxin M1 Rapid Test Kit(0.03ppb)

Rapid Test for the presence of Aflatoxin M₁ in raw cow milk

BACKGROUND

Aflatoxin M₁ is a chemical compound of the aflatoxin class, a group of mycotoxins produced by three species of Aspergillus - Aspergillus flavus, Aspergillus parasiticus, and the rare Aspergillus nomius - which contaminate plant and plant products.

Aspergillus flavus produces only B-type aflatoxins. Aflatoxin M₁ is the hydroxylated metabolite of aflatoxin B₁ and can be found in milk or milk products obtained from livestock that have ingested contaminated feed. The carcinogenic potency of aflatoxin M₁ in sensitive species is about one order of magnitude less than that of aflatoxin B₁. Aflatoxin M₁ is usually considered to be a detoxication by-product of aflatoxin B₁. The main sources of aflatoxins in feeds are peanut, meal, maize and cottonseed meal

According to the review conducted by the Dutch National Institute for Public Health and the Environment (RIVM) on behalf of the Food and Agriculture Organization (FAO) approximately 60 countries have set specific limits for aflatoxin M₁. The European Union countries generally apply a maximum level of 0.05 μg/kg milk. Some countries in Africa, Asia and Latin America also propose this level. In contrast, the USA as well as some European and several Asian countries accept a maximum level of 0.5μg/kg aflatoxin M₁ in milk, which is also the harmonized MERCOSUR limit applied in Latin America. The 0.5μg/kg limit for aflatoxin M₁.

PRECAUTIONS

l The milk sample should has NO agglomeration, mix evenly before assay.

l Frozen milk sample can be stored at -20℃ for 2 months, thawed milk sample can be stored for 24 hours, discard the left unused thawed milk sample.

l Frozen milk sample should be thawed with 40-60℃ watar bath or naturally to ambient temperature.

l Do the assay following the instruction, do NOT touch the membrane of the dipstick.

l Seal the bottle after taking out required dipsticks.

l The dipstick is used for only once; Do NOT use it repeatedly.

l The positive result should be further confirmed.

PRINCIPLE

The WatarBio DairyPal^® AFL M₁ is competitive test involving one receptors in one single operation, the aflatoxin M₁ in the raw milk sample competes for the antibody with the antigen coated on the membrane of the test dipstick. Then after a color reaction, when the sample is free of aflatoxin M₁, a strong colour development occurs at the specific capture lines, indicating the absence of the concerned analytes in the milk sample. On the contrary, the presence of aflatoxin M₁ in the sample will not cause the strong coloured signal to appear at the specific capture lines.

APPLICATIONS

The WatarBio DairyPal^® AFL M₁ is a rapid test that detect simultaneously the presence of the Aflatoxin M₁ molecules in raw cow milk.

LIMIT OF DETECTION (LOD)

0.03 μg/L(ppb)

CONTENTS

INSTRUMENT MAY NEEDED BUT NOT PROVIDED

l DairyPal^® Dry incubator(Model: Mini B)

l DairyPal^® Dipstick Reader (Model: TSR-100A, OPTIONAL)

TEST PROCEDURE

1. Read the instruction attentively, choose a clean and dry place to perform the test, wash and dry your hands before starting.

2. Take the kit out of the fridge and wait until the temperature of the reagents reaches the ambient temperature.

3. Calculate how many milk sample will be tested and mark the sample tubes to identify. The milk samples should be fully liquid without any clots or deposition, the ideal temperature of milk sample is between 4 to 20℃.

4. Take the tubes required from the kit package, take out as many micro-wells and dipsticks as the milk samples to be tested, and make proper marks to match the milk sample.

l To open a dipsticks tube, get the tube cap off with your thumb.

l Recover the cap tightly after taking out the required micro-wells and dipsticks.

l Empty one test tube then open another one.

l Keep the unused micro-well caps tightly sealed. Do NOT tear off the strip of 8 microwell caps.

l Use the exposed dipsticks in 1 hour.

5. Preheat the Dry Incubator(suggest to use DairyPal^® Mini-B Dry Incubator) to 40℃.

6. Place the required numbers of the mircrowells in the dry incubator, transfer 200ul of the milk samples into the microwells, inject and reject the milk sample for 5 times consecutively to homogenize the lyophilized reagent and milk sample.

l Dip the end of the plastic pipette(DairyPal^® Mini pipette) tips into the milk and slowly releases the sucker. When the sucker is back into its initial position, transfer the 200µl of milk into the reagents, by slowly pushing the sucker down to the bottom.

7. Start the timer to incubate for 3 minutes at 40℃;

l During the incubation, The receptors will detect the presence of the antibiotics in the milk sample. The reaction will take 3 minutes.

8. Dip the dipsticks into the micro-wells with the "Sample End" down side to the bottom.

9. Start the timer to incubate for 5 minutes at 40℃ again.

10. Then take out the dipsticks and place them laterally in a dry clean place, peel off the sample pad, interpret the result.

l Use a clean nipper(or new pipette tip) to scrape gently and slowly the sample pad.

l The peeling off of the sample pad will stop the ongoing reaction of the receptors.

l File the used sample pad peeled off dipsticks if necessary.

RESULTS INTERPRETATION

The dipsticks are embodied with 2 lines, Control line and Test line, which are briefly expressed as “CTL” and “T”. The test results will be determined by the color density of the lines. The following diagram describes the result identification.

l NEGATIVE: Line CTL and Line T both are visible, Line T is stronger than or equal to Line CTL. which means that, the milk sample contains aflatoxin M₁ fewer than the detection limit.

l POSITIVE: Line CTL is visible, Line T is invisible or weaker than Line CTL, which means that the milk sample contains the aflatoxin M₁ more than detection limit.