SC066 Beta-lactams tetracyclines Stremptomycin Chloramphenicol Rapid Test Kit

Product Item : SC066
Category： 4 in 1 Test Kit
Detection Time:9min(10min+7min)
Detection Limit:1.5-500ppb
Detection Temp: Room Temperature
Shelf Life:1 year
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DairyPal^® BTSC Art. No.: SC066-96T

ß-lactams & Tetracyclines & Streptomycin & Chloramphenicol 4in1 Rapid Test Kit

PRINCIPLE

The WatarBio DairyPal^® BTSC is competitive test involving four receptors in one single operation, the ß-lactams and Tetracyclines and Streptomycin and Chloramphenicol in the milk sample compete for the antibody with the antigen coated on the membrane of the test dipstick. Then after a color reaction, when the sample is free of the analytes, a strong colour density development occurs at the specific capture lines, indicating the presence of the concerned analytes in the milk sample. On the contrary, the presence of the analytes in the sample will not cause the strong coloured signal at the specific capture lines.

APPLICATIONS

The WatarBio DairyPal^® BTSC is a rapid test that detects simultaneously the presence of the ß-lactams and Tetracyclines and Streptomycin and Chloramphenicol molecules in raw cow milk.

LIMIT OF DETECTION (LOD)

Antibiotics		LOD(μg/L)	MRL(μg/L)
β-lactams	Penicillin G	1-1.5	4
	Ampicillin	2-3	4
	Amoxicillin	4	4
	Oxacillin	4	30
	Cloxacillin	3	30
	Dicloxacillin	5	30
	Nafcillin	10-12	30
	Benzathine Benzylpenicillin	2	-
	Procaine penicillin	2	4
Cephalosporins	Cefquinome	6-10	20
	Cefacetrile	15	125
	Cefalonium	2-3	20
	Cefoperazone	2	50
	Cefapirin	6	60
	Ceftiofur	90	100
	Cefuroxime	150	-
	Cefalexin	500	100
	Cefazolin	30	50
Tetracyclines	Tetracycline	10	100
	Oxytetracycline	10	100
	Doxycycline	10	100
	Chlortetracycline	10	100
Aminoglycoside	Streptomycin	40-80	200
Aminoglycoside	Dihydrostreptomycin Sulphate	40-80	200
	Chloramphencol	0.3	0

CONTENTS

96 Test dipsticks : 12 tubes each with 1 strip of 8 reagent microwells and 8 dipsticks

1 Negative Control

1 Positive Control

1 Microwell holder

1 Mini Pipette

1 Kit insert

100pcs pipette tips

INSTRUMENT MAY NEEDED BUT NOT PROVIDED

l DairyPal^® Dipstick Reader (Model: TSR-100A, OPTIONAL)

TEST PROCEDURE

1. Read the instruction attentively, choose a clean and dry place to perform the test, wash and dry your hands before starting.

2. Take the kit out of the fridge and wait until the temperature of the reagents reaches the ambient temperature.

3. Calculate how many milk sample will be tested and mark the sample tubes to identify. The milk samples should be fully liquid without any clots or deposition, the ideal temperature of milk sample is between 4 to 20℃.

4. Take the tubes required from the kit package, take out as many micro-wells and dipsticks as the milk samples to be tested, and make proper marks to match the milk sample.

l To open a dipsticks tube, press the safety ring down, take off the ring and get the tube cap off with your thumb.

l Recover the cap tightly after taking out the required micro-wells and dipsticks.

l Empty one test tube then open another one.

l Keep the unused micro-well caps tightly sealed. Do NOT tear off the strip of 8 microwell caps.

l Use the exposed dipsticks in 1 hour.

5. Place the required numbers of the mircrowells in the microwell holder, transfer 200ul of the milk samples into the microwells, inject and reject the milk sample for 5 times consecutively to homogenize the lyophilized reagent and milk sample.

l Dip the end of the plastic pipette(DairyPal^® Mini pipette) tips into the milk and slowly releases the sucker. When the sucker is back into its initial position, transfer the 200µl of milk into the reagents, by slowly pushing the sucker down to the bottom.

6. Start the timer to incubate for 3 minutes at ambient temperature(20-35℃).

l During the incubation, The receptors will detect the presence of the antibiotics in the milk sample. The reaction will take 3 minutes.

7. Dip the dipsticks into the micro-wells with the "Sample End" down side to the bottom.

8. Start the timer to incubate for 7 minutes at ambient temperature(20-35℃).

9. Then take out the dipsticks and place them laterally in a dry clean place, peel off the sample pad, interpret the result.

l Use a clean nipper(or new pipette tip) to scrape gently and slowly the sample pad.

l The peeling off of the sample pad will stop the ongoing reaction of the receptors.

l File the used sample pad peeled off dipsticks if necessary.

RESULTS INTERPRETATION

The dipsticks are embodied with 5 lines, Control line, β-lactams Line, Tetracylcines Line, Streptomycin Line and Chloramphenicol Line, which are briefly expressed as “CTL”, “B” ,“T” _,“S” and “C”. The test results will be determined by the color presence of the lines. The following diagram describes the result identification.

l NEGATIVE: Line CTL, B, T, S and C are all visible, color of Line B, T, S and C are all stronger than Line CTL, which means that, the milk sample contains fewer antibiotics than value given in the table.

l β-lactams POSITIVE: Line CTL is visible, Line B is weaker than or equal to Line CTL, Line T_, S and C are all stronger than Line CTL, which means that the milk sample contains the ß-lactams more than the value in the table, the tetracyclines, streptomycin and chloramphenicol fewer that the value in the table.