SC053 Cow Milk/ Tylosin and Tilmicosin Milk Antibiotics Test Kit

PRINCIPLE

The DairyPal^® TYL is a competitive test involving one receptor in one single operation. The Tylosin and Tilmicosin antibiotics in the milk sample compete for the antibody with the antigen coated on the membrane of the test dipstick. Then after a color reaction, when the sample is free of antibiotics, a strong colour development occurs at the specific capture line, indicating the absence of the concerned analytes in the milk sample. On the contrary, the presence of antibiotics in the sample will not cause the strong coloured signal to appear at the specific capture lines.

APPLICATIONS

The DairyPal^® TYL is a rapid test that detects simultaneously the presence of Tylosin and Tilmicosin molecules in a raw cow milk, pasteurized milk, UHT milk, milk powder (dissolve the sample with water at the vol. ration of 1:9) sample.

LIMIT OF DETECTION (LOD)

● Tylosin:5 μg/L(ppb)

● Tilmicosin:5 μg/L(ppb)

INSTRUMENT MAY NEEDED BUT NOT PROVIDED

l DairyPal^® Dry incubator (Model: Mini B)

l DairyPal^® Dipstick Reader(Model: TSR-100A, OPTIONAL)

TEST PROCEDURE

1. Read the instruction attentively, choose a clean and dry place to perform the test, wash and dry your hands before starting.

2. Take the kit out of the fridge and wait until the temperature of the reagents reaches the ambient temperature.

3. Calculate how many milk samples will be tested and mark the sample tubes to identify. The milk samples should be fully liquid without any clots or deposition. The ideal temperature of milk sample is between 4 to 20℃.

4. Take the tubes required from the kit package, take out as many micro-wells and dipsticks as the milk samples to be tested, and make proper marks to match the milk sample.

l To open a dipsticks tube, press the safety ring down, take off the ring and get the tube cap off with your thumb.

l Recover the cap tightly after taking out the required micro-wells and dipsticks.

l Empty one test tube then open another one.

l Keep the unused micro-well caps tightly sealed. Do NOT tear off the strip of 8 microwell caps.

l Use the exposed dipsticks in 1 hour.

5. Preheat the Dry Incubator(suggest to use DairyPal^® Mini-B Dry Incubator) to 35℃.

6. Place the required numbers of the mircrowells in the dry incubator, transfer 200ul of the milk samples into the microwells, inject and reject the milk sample for 5 times consecutively to homogenize the lyophilized reagent and milk sample.

l Dip the end of the plastic pipette (DairyPal^® Mini pipette) tips into the milk and slowly releases the sucker. When the sucker is back into its initial position, transfer the 200µl of milk into the reagents by slowly pushing the sucker down to the bottom.

7. Start the timer to incubate for 5 minutes at 35℃;

l During the incubation, the receptors will detect the presence of the antibiotics in the milk sample. The reaction will take 5 minutes.

8. Dip the dipsticks into the micro-wells with the "Sample End" down side to the bottom.

9. Start the timer to incubate for 5 minutes at 35℃ again.

10. Then take out the dipsticks and place them laterally in a dry clean place, peel off the sample pad, interpret the result.

l Use a clean nipper (or new pipette tip) to scrape gently and slowly the sample pad.

l The peeling-off of the sample pad will stop the ongoing reaction of the receptors.

l File the used sample pad, peeled-off dipsticks if necessary.