SC060 Beta-lactams Tetracyclines Sulfonamides Rapid Test Kit

Product Item : SC060
Category： 3 in 1 Test Kit
Detection Time:8min(3min+5min)
Detection Limit:0.3-100ppb
Detection Temp:ambient temp. or 25℃
Shelf Life:1 year
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Beta-lactams Tetracyclines Sulfonamides BTS Rapid Test Kit

PRINCIPLE

The Watarbio DairyPal^® TSB is competitive test involving three receptors in one single operation, the tetracyclines, sulfonamides and β-lactams antibiotics in the milk sample compete for the antibody with the antigen coated on the membrane of the test dipstick. Then after a color reaction, when the sample is free of antibiotics, a strong colour development occurs at the specific capture lines, indicating the absence of the concerned analytes in the milk sample. On the contrary, the presence of antibiotics in the sample will not cause the strong coloured signal to appear at the specific capture lines.

APPLICATIONS

The DairyPal^® TSB is a rapid test that detect simultaneously the presence of the tetracyclines, sulfonamides and β-lactams molecules in raw cow/goat milk.

LIMIT OF DETECTION (LOD)

Antibiotics		LOD(μg/L)	MRL(μg/L)
β-lactams	Penicillin G	1.5-2	4
	Ampicillin	3-4	4
	Amoxicillin	4-5	4
	Oxacillin	6-8	30
	Cloxacillin	6-8	30
	Dicloxacillin	6-8	30
	Nafcillin	20-30	30
Cephalosporins	Cefquinome	20-30	20
	Cefacetrile	40	125
	Cefalonium	5-10	20
	Ceftriaxone	100	-
	Cefoperazone	40-50	50
	Cephapirin	20	60
	Ceftiofur	90-100	100
Sulfonamides	Sulfamethazine	2-2.5	100
	Sulfadimethoxine	1-1.2	100
	Sulfaquinoxaline	0.8-1	100
	Sulfadiazine	0.4-0.5	100
	Sulfapyridine	18-20	100
	Sulfamonomethoxine	0.3-0.4	100
	Sulfachlorpyridazine	1.5-2	100
	Sulfamethoxypyridazine	8-9	100
	Sulfamerazine	1-1.5	100
	Sulfathiazole	3-4	100
Tetracyclines	Tetracycline	40-60	100
	Oxytetracycline	80-100	100
	Doxycycline	40-60	100
	Chlortetracycline	40-60	100

CONTENTS

l 12 tubes each with 1 strip of 8 reagent microwells and 8 dipsticks.

l 1 Negative Control

l 1 Positive Control

l 1 Microwell holder

l 1 Mini Pipette

l 1 Kit insert

l 100pcs Disposable plastic pipette

INSTRUMENT MAY NEEDED BUT NOT PROVIDED

l DairyPal^® Dipstick Reader (Model: TSR-100A, Optional)

l DairyPal^® Dry incubator(Model: Mini B, Optional)

TEST PROCEDURE

1. Read the instruction attentively, choose a clean and dry place to perform the test, wash and dry your hands before starting.

2. Take the kit out of the fridge and wait until the temperature of the reagents reaches the ambient temperature.

3. Calculate how many milk sample will be tested and mark the sample tubes to identify. The milk samples should be fully liquid without any clots or deposition, the ideal temperature of milk sample is between 4 to 20℃.

4. Take the tubes required from the kit package, take out as many micro-wells and dipsticks as the milk samples to be tested, and make proper marks to match the milk sample.

l To open a dipsticks tube, press the safety ring down, take off the ring and get the tube cap off with your thumb.

l Recover the cap tightly after taking out the required micro-wells and dipsticks.

l Empty one test tube then open another one.

l Keep the unused micro-well caps tightly sealed. Do NOT tear off the strip of 8 microwell caps.

l Use the exposed dipsticks in 1 hour.

5. Preheat the Dry Incubator(suggest to use DairyPal^® Mini-B Dry Incubator) to 25℃.

6. Place the required numbers of the mircrowells in the dry incubator, transfer 200ul of the milk samples into the microwells, inject and reject the milk sample for 5 times consecutively to homogenize the lyophilized reagent and milk sample.

l Dip the end of the plastic pipette(DairyPal^® Mini pipette) tips into the milk and slowly releases the sucker. When the sucker is back into its initial position, transfer the 200µl of milk into the reagents, by slowly pushing the sucker down to the bottom.

7. Start the timer to incubate for 3 minutes at 25℃ or ambient temperature;

l During the incubation, The receptors will detect the presence of the antibiotics in the milk sample. The reaction will take 3 minutes.

8. Dip the dipsticks into the micro-wells with the "Sample End" down side to the bottom.

9. Start the timer to incubate for 6 minutes at 25℃ or ambient temperature again.

10. Then take out the dipsticks and place them laterally in a dry clean place, peel off the sample pad, interpret the result.

l Use a clean nipper(or new pipette tip) to scrape gently and slowly the sample pad.

l The peeling off of the sample pad will stop the ongoing reaction of the receptors.

l File the used sample pad peeled off dipsticks if necessary.

RESULTS INTERPRETATION

The dipsticks are embodied with 4 lines, Control line, Tetracyclines line, Sulfonamides line and β-lactams line, which are briefly expressed as “CTL”, “T”,”S” and “B”. The test results will be determined by the color density of the lines. The following diagram describes the result identification.

Method A (Visual Interpretation)

l NEGATIVE: Line CTL is visible, Line T, Line S and Line B are all stronger than or equal to Line CTL, which means that, the milk sample contains fewer tetracyclines, sulfonamides and β-lactams than value given in the table.

l Tetracyclines POSITIVE: Line CTL is visible, Line T is weaker than Line CTL, Line S and Line B both are stronger than Line CTL, which means that the milk sample contains the tetracyclines more than the value in the table, the sulfonamides and β-lactams fewer that the value in the table.